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Related Concept Videos

Transgenic Organisms00:53

Transgenic Organisms

Overview
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Related Experiment Video

Updated: Jul 10, 2026

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
09:48

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Published on: August 21, 2010

Transgenic pig expressing the enhanced green fluorescent protein produced by nuclear transfer using

Liangxue Lai1, Kwang-Wook Park, Hee-Tae Cheong

  • 1Department of Animal Sciences, University of Missouri-Columbia, Columbia, Missouri 65211, USA.

Molecular Reproduction and Development
|July 12, 2002
PubMed
Summary
This summary is machine-generated.

Researchers created a healthy transgenic piglet expressing enhanced green fluorescent protein (EGFP) using somatic cell nuclear transfer. This method offers an alternative for producing genetically modified pigs for future research applications.

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Area of Science:

  • Reproductive Biology
  • Genetics and Genomics
  • Biotechnology

Background:

  • Somatic cell nuclear transfer (SCNT) is a key technique for producing genetically modified animals.
  • Producing transgenic pigs efficiently remains a challenge, necessitating alternative methods.
  • Enhanced green fluorescent protein (EGFP) is a widely used marker for genetic modification studies.

Purpose of the Study:

  • To develop an alternative method for producing transgenic pigs using SCNT.
  • To evaluate the efficiency of using colchicine-treated, EGFP-transduced fetal fibroblasts as donor cells for SCNT.
  • To assess the developmental competence and EGFP expression in reconstructed embryos and the resulting piglet.

Main Methods:

  • Fetal fibroblast cells were transduced with replication-defective vectors encoding EGFP.
  • Transduced cells were synchronized to the G2/M stage using colchicine.
  • Nuclear transfer was performed by transferring donor nuclei into enucleated porcine oocytes, followed by fusion and activation.
  • Reconstructed embryos were cultured in vitro, and developmental parameters and EGFP expression were monitored.
  • In vivo development was assessed by transferring reconstructed embryos into surrogate gilts.

Main Results:

  • Nuclear envelope breakdown and premature chromosome condensation occurred within 2 hours post-activation.
  • Pronuclear formation was observed in 62.5% of reconstructed oocytes by 12 hours.
  • Approximately 77.2% of day 5 embryos were diploid, and 9.4% developed to the blastocyst stage.
  • EGFP expression was detected early in reconstructed oocytes and embryos, with sustained expression in blastocysts (71.5%) and the delivered piglet.
  • Three out of four surrogate gilts became pregnant, with one delivering a healthy EGFP-expressing transgenic piglet.

Conclusions:

  • The combination of EGFP transduction of somatic cells and nuclear transfer of colchicine-treated donor cells is a viable alternative for producing transgenic pigs.
  • This method facilitates the generation of genetically marked pigs for future research, particularly in studies requiring genetically marked cells.
  • The successful production of a live transgenic piglet demonstrates the potential of this SCNT approach for advancing genetic engineering in swine.