Related Experiment Video
Updated: Sep 30, 2026
Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
Published on: February 9, 2016
Na+/H+ exchange in primary, secondary and n-butyrate-treated cultures of ruminal epithelial cells: short
P Gálfi1, Susan Neogrády, G Gäbel
1Department of Physiology and Biochemistry, Faculty of Veterinary Science, Szent István University, H-1400 Budapest, P.O. Box 2, Hungary. pgalfi@mail.univet.hu
Abstract:
Rate of amiloride-sensitive Na+ uptake into cultured rumen epithelial cells was studied in order to clarify the influence of culture conditions on Na+/H+ exchange (NHE). Cell cultures were exposed to Na-n-butyrate or not for seven days or subcultured. On the 14th day of culturing, primary cell cultures without butyrate exposure showed both non-stratified and stratified growth. Na-n-butyrate treated 14-day-old cultures and 3-day-old subcultures contained mostly non-stratified, i.e. non-keratinised cells. Both n-butyrate treatment and subculturing increased total and amiloride-sensitive Na+ uptake. Our results indicate that Na+ uptake via NHE is determined by the amount and the ratio of non-stratified (non-keratinised) cells.
