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Updated: Sep 11, 2026
Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays
Published on: September 26, 2011
[HCV RNA assessment by PCR technique for screening post-transfusion HCV infection among blood donors]
Wenge Xing1, Hongyi Xu, Rong Ma
1National Center for Clinical Laboratories, Beijing Hospital, Beijing 100730, China.
Objective:
To survey the application of PCR for screening HCV RNA from blood donations within the window period.
Methods:
According to a standardized method, 12 blood banks organized by the National Center for Clinical Laboratories collected and prepared about ten thousands specimens. The specimens were tested with two different kits.
Results:
Among the 7173 specimens A group, 21 were PCR positive for HCV RNA. The positive rate was 0.29%. There were not positive for HCV RNA among 7477 specimens (B group).
Conclusions:
It is feasible to use the PCR screening for the detection of HCV RNA of blood donations but is unnecessary to standardize the specimen collection and the kit selection.
