Jove
Visualize
Contact Us

Related Experiment Videos

Isolating vessels from the mouse brain for gene expression analysis using laser capture microdissection.

Helen J Ball1, Brent McParland, Catherine Driussi

  • 1Department of Pathology, Blackburn Bldg. D06, University of Sydney, Camperdown N.S.W. 2006, Australia. helenb@med.usyd.edu.au

Brain Research. Brain Research Protocols
|July 13, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Applicability of Redirecting Artemisinins for New Targets.

Global challenges (Hoboken, NJ)·2023
Same author

Results from the AAPM Task Group 324 respiratory motion management in radiation oncology survey.

Journal of applied clinical medical physics·2022
Same author

Investigating potential TRPV1 positive feedback to explain TRPV1 upregulation in airway disease states.

Drug development and industrial pharmacy·2022
Same author

Editorial: The Relationships Between Infectious Agents and Dementia.

Frontiers in cellular neuroscience·2022
Same author

Efficient Treatment of Experimental Cerebral Malaria by an Artemisone-SMEDDS System: Impact of Application Route and Dosing Frequency.

Antimicrobial agents and chemotherapy·2021
Same author

Double deficiency of toll-like receptors 2 and 4 alters long-term neurological sequelae in mice cured of pneumococcal meningitis.

Scientific reports·2019
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Researchers developed a new method to isolate brain vessels for gene expression analysis. This technique allows studying changes in blood-brain barrier cells during cerebral malaria pathogenesis.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pathology

Background:

  • Gene expression studies typically use bulk tissue RNA, masking cell-specific changes.
  • Understanding the blood-brain barrier's role in cerebral malaria requires cell-specific analysis.

Purpose of the Study:

  • To develop and validate a method for isolating brain vessels for gene expression analysis.
  • To investigate gene expression changes within the blood-brain barrier during cerebral malaria.

Main Methods:

  • Utilized laser capture microdissection to isolate brain vessels from mouse tissue.
  • Employed alkaline phosphatase staining for vessel visualization.
  • Validated vessel enrichment by measuring endothelial cell and pericyte-specific markers.
  • Quantified indoleamine 2,3-dioxygenase mRNA to assess malaria-induced changes.

Related Experiment Videos

Main Results:

  • Isolated RNA was highly enriched for endothelial cell and pericyte-specific mRNAs.
  • Successfully detected malaria-induced changes in gene expression within isolated vessels.
  • Demonstrated the feasibility of studying gene expression changes at the blood-brain barrier.

Conclusions:

  • Laser capture microdissection is an effective method for isolating brain vessels for targeted gene expression studies.
  • This approach enables the investigation of molecular changes within the blood-brain barrier during disease pathogenesis.
  • Provides a novel tool for understanding cerebral malaria at the cellular level.