Related Experiment Videos
Base pairing properties of 8-oxo-7,8-dihydroadenosine in cDNA synthesis by reverse transcriptases
Sang Kook Kim1, Ji Young Kim, Ae Kyeong Baek
1Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu 702-701, Republic of Korea.
Bioorganic & Medicinal Chemistry Letters
|July 13, 2002
Abstract:
Incorporation of nucleotides opposite 8-oxo-7,8-dihydroadenosine (8-oxoA) in oligonucleotides with dNTPs by three reverse transcriptases (AMV-, MMRV-, RAV2-RT) in cDNA synthesis was studied. Guanine as well as thymine was incorporated preferentially by all reverse transcriptases. In the melting temperature experiment, 8-oxoA and 8-oxoA-Me formed base pairs with thymine and guanine with similar stabilities.