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Updated: Sep 30, 2026

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE
Published on: October 16, 2017
A partially 3-O-methylated (1-->4)-linked alpha-D-galactan and alpha-D-mannan from Pleurotus ostreatoroseus Sing
Fábio R Rosado1, Elaine R Carbonero, Carlos Kemmelmeier
1Departamento de Bioquímica, Universidade Estadual de Maringá, Av. Colombo 5790, Brazil.
Abstract:
The two main water-soluble extracellular polysaccharides produced by the basidiomycete fungus Pleurotus ostreatoroseus Sing were isolated and purified. They were characterized using 13C, 1H, and 1H, 13C HMQC NMR spectroscopy, methylation analysis, and Smith degradation. One was a mannan having a main chain of (1-->6)-linked alpha-D-mannopyranosyl residues, almost all of which were branched at O-2 with side chains of different lengths, containing 2-O- and 3-O-linked mannopyranosyl units. The other was a partially 3-O-methylated (1-->4)-linked alpha-D-galactopyranan, a structure that has not been previously described.
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