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Published on: August 13, 2019
P2X(3) receptor subunit messenger RNA expression in the female mouse bladder after oophorectomy with or without
Michael E Carley1, William A Cliby, Thomas C Spelsberg
1Departments of Obstetrics and Gynecology, Division of Gynecological Surgery, Mayo Clinic, Rochester, MN 55905, USA. carley.michael@mayo.edu
Objective:
This investigation was undertaken to determine whether the sensory neuron adenosine triphosphate receptor subunit (P2X(3)) messenger RNA expression is altered in female mouse bladders after surgical oophorectomy with or without estrogen replacement.
Study Design:
The mean relative concentrations of the P2X(3) receptor in 30 female mouse bladders (10 sham operated, 10 oophorectomized, and 10 oophorectomized with estrogen replacement) were determined with quantitative reverse transcription-polymerase chain reaction analysis.
Results:
P2X(3) expression increased after surgical oophorectomy (0.91 +/- 0.16 vs 1.04 +/- 0.11, P =.048). However, P2X(3) expression after oophorectomy and immediate estrogen replacement did not differ from that of oophorectomy alone (1.11 +/- 0.15 vs 1.04 +/- 0.11, P =.206).
Conclusions:
The P2X(3) sensory neuron receptor messenger RNA expression is increased after oophorectomy but is not influenced by subsequent estrogen replacement. This has clinical significance because sensory neuron receptors may be associated with certain forms of bladder dysfunction.
Insights
Surgical oophorectomy in female mice increased sensory neuron P2X3 receptor mRNA expression. Estrogen replacement did not alter this P2X3 expression, suggesting a role in bladder dysfunction.
Area of Science:
- Urology
- Neuroscience
- Endocrinology
Background:
- Sensory neuron receptors play a role in bladder function.
- Oophorectomy can alter physiological processes in female mammals.
Purpose of the Study:
- To investigate the effect of oophorectomy and estrogen replacement on P2X3 receptor mRNA expression in female mouse bladders.
Main Methods:
- Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used.
- Analysis was performed on 30 female mouse bladders: sham-operated, oophorectomized, and oophorectomized with estrogen replacement.
Main Results:
- P2X3 receptor mRNA expression was significantly increased in bladders after oophorectomy.
- Estrogen replacement following oophorectomy did not significantly alter P2X3 receptor mRNA levels compared to oophorectomy alone.
Conclusions:
- Oophorectomy leads to increased P2X3 sensory neuron receptor mRNA expression in female mouse bladders.
- Estrogen replacement does not influence this P2X3 expression.
- Elevated P2X3 expression may be linked to bladder dysfunction.
