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Updated: Jul 16, 2026

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
Published on: April 13, 2015
Telomere Shortening of MCF-7 Cells Caused by Antisense Telomerase cDNA
Xiao-Wei Zhang1, Hai-Ni Liao, Tan-Jun Tong
1Department of Biochemistry and Molecular Biology, Beijing Medical University, Beijing 100083, China. biochem@mail.bjmu.edu.cn
Abstract:
pAdE(1)CMVITREXneo is a novel adenovirus vector which can integrate foreign genes into genome of host cells. The cDNA of human telomerase RNA was integrated inversely into the vector via antisense oligonucleotide technique. Antisense recombinant virus (vAdT-AAV) was obtained by cotransfecting pAdT and pBHG(11) into 293 cells. The telomere length of breast cancer MCF-7 cells was found shortened after the transfection by vAdT-AAV.
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