Related Experiment Video
Updated: Mar 23, 2026

Transient Expression of Foreign Genes in Insect Cells sf9 for Protein Functional Assay
Published on: February 22, 2018
Human &mgr; Opioid Receptor Expressed in Sf9 Insect Cells was Functionally Coupled to Endogenous G Protein
Qiang Wei1, Tie-Lin Wang, Jie Chen
1Shanghai Institute of Materia Medica, the Chinese Academy of Sciences, Shanghai 200031, China. dhzhou@server.shcnc.ac.cn
Abstract:
Human &mgr; opioid receptor(H&mgr;OR) with a tag of six consecutive histidines at its carboxyl terminus overexpressed in recombinant baculovirus infected Sf9 insect cells was demonstrated to be functionally coupled to endogenous G proteins. Na(+) and GTP could reduce the affinity binding of etorphine and Ohmefentanyl(Ohm) to H&mgr;OR overexpressed in Sf9. The binding of (35)S GTPgammaS to Sf9 cell membranes containing H&mgr;OR was stimulated by DAGO and Ohm. This stimulation could be blocked by naloxone or the pretreatment of PTX. Also, DAGO and Ohm could inhibit the increasing of intracellular cAMP stimulated by forskolin. The results showed H&mgr;OR expressed in Sf9 insect cells were functionally coupled to endogenous PTX sensitive G(i/o) proteins.
Related Concept Videos
Opioid Receptors: Overview
Transducer Mechanism: G Protein–Coupled Receptors
GPCRs are also called heptahelical,...
G Protein-coupled Receptors
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
G-protein Coupled Receptors
G-Protein Gated Ion Channels
Sensory...
Activation and Inactivation of G Proteins

