Activity of Lipase in Reversed Micelles

The enzyme activity of lipase from Candida rugosa in AOT isooctane reversed micelles was investigated. Effect of pH, temperature, substrate concentration, waterpool (R value), choice of organic solvent, surfactant concentration on the activity of lipase were studied in detail. Results indicate that the enzyme in reversed micelles follows the Michaelis-Menten dynamics temperature optimum=32.5 degrees, pH optimum=7.0, and maximum activity is reached at the concentration of olive oil of 40%. The enzyme activity was dependent on R (molar ratio of water to surfactant) and maximum activity was obtained at R=11.