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Separation and Characterization of Oligodeoxynucleotides by FPLC
Hui-Wei Tang1, You-Xin Jin, De-Xu Zhu
1State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry, the Chinese Academy of Sciences, Shanghai 200031, China. yxjin@sunm.shcnc.ac.cn
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao Acta Biochimica Et Biophysica Sinica
|July 13, 2002
Abstract:
The purity of the antisense drugs synthesized by DNA synthesizer should be determined effectively. At pH 12,the oligodeoxynucleotides (ODNs) ranged from 19 bases to 21 bases can be separated successfully on the anion exchange column MONO-Q by elution with a NaCl gradient. The experiments proved that the fast protein liquid chromatography (FPLC) was a useful tool for isolation and identification of the antisense drugs.