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Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
Published on: March 14, 2019
Microsatellite instability and mutation analysis of candidate genes in unselected sardinian patients with endometrial
Paola Baldinu1, Antonio Cossu, Antonella Manca
1Institute of Molecular Genetics, C.N.R., Località Tramariglio, Santa Maria La Palma (Sassari), Italy.
Background:
Microsatellite instability (MSI) is due mostly to a defective DNA mismatch repair (MMR). Inactivation of the two principal MMR genes, hMLH1 and hMSH2, and the PTEN tumor suppressor gene seems to be involved in endometrial tumorigenesis. In this study, Sardinian patients with endometrial carcinoma (EC) were analyzed to assess the prevalence of both the mutator phenotype (as defined by the presence of MSI and abnormal MMR gene expression at the somatic level) and the hMLH1, hMSH2, and PTEN germline mutations among patients with MSI positive EC.
Methods:
Paraffin embedded tissue samples from 116 consecutive patients with EC were screened for MSI by polymerase chain reaction-based microsatellite analysis. Immunohistochemistry (IHC) with anti-hMLH1 and anti-hMSH2 antibodies was performed on MSI positive tumor tissue sections. Germline DNA was used for mutational screening by denaturing high-performance liquid chromatography analysis and automated sequencing.
Results:
Thirty-nine patients with EC (34%) exhibited MSI; among them, 25 tumor samples (64%) showed negative immunostaining for hMLH1/hMSH2 proteins (referred to as IHC negative). No disease-causing mutation within the coding sequences of the hMLH1/hMSH2 and PTEN genes was found in patients with EC who had the mutator phenotype (MSI positive and IHC negative), except for a newly described hMLH1 missense mutation, Ile655Val, that was observed in 1 of 27 patients (4%). Although MSI was more common among patients with advanced-stage EC and increased as the tumor grade increased, no significant correlation with disease free survival or overall survival was observed among the two groups (MSI positive or MSI negative) of patients with EC.
Conclusions:
In patients with MSI positive EC, epigenetic inactivations rather than genetic mutations of the MMR genes seem to be involved in endometrial tumorigenesis. No prognostic value was demonstrated for MSI in patients with EC.
Insights
Microsatellite instability (MSI) in endometrial cancer (EC) is often due to epigenetic changes in DNA mismatch repair (MMR) genes, not mutations. MSI did not prove to have prognostic value for EC patients in this study.
Area of Science:
- Oncology
- Genetics
- Molecular Biology
Background:
- Microsatellite instability (MSI) arises from defective DNA mismatch repair (MMR), frequently impacting endometrial tumorigenesis via hMLH1, hMSH2, and PTEN genes.
- This study investigated MSI prevalence, MMR gene expression, and germline mutations in Sardinian endometrial carcinoma (EC) patients.
Purpose of the Study:
- To assess the prevalence of the mutator phenotype in EC patients.
- To identify germline mutations in hMLH1, hMSH2, and PTEN genes in MSI-positive EC patients.
Main Methods:
- 116 EC patient samples were analyzed for MSI using polymerase chain reaction.
- Immunohistochemistry (IHC) assessed hMLH1/hMSH2 protein expression in MSI-positive tumors.
- Germline DNA underwent mutational screening via denaturing high-performance liquid chromatography and automated sequencing.
Main Results:
- 34% of EC patients (39/116) exhibited MSI; 64% of these (25/39) were IHC negative for hMLH1/hMSH2.
- No significant disease-causing mutations in hMLH1, hMSH2, or PTEN were found in MSI-positive, IHC-negative patients, except for one novel hMLH1 missense mutation (Ile655Val).
- MSI correlated with advanced EC stage and higher tumor grade but lacked prognostic value for disease-free or overall survival.
Conclusions:
- Epigenetic inactivation of MMR genes, rather than genetic mutations, appears central to tumorigenesis in MSI-positive EC.
- MSI status does not demonstrate prognostic significance for endometrial carcinoma patients.
