Related Experiment Video
Updated: Sep 30, 2026
BS3 Chemical Crosslinking Assay: Evaluating the Effect of Chronic Stress on Cell Surface GABAA Receptor Presentation in the Rodent Brain
Published on: May 26, 2023
Psychological stress, but not physical stress, causes increase in diazepam binding inhibitor (DBI) mRNA expression in
Masashi Katsura1, Yutaka Mohri, Keijiro Shuto
1Department of Pharmacology, Kawasaki Medical School, Matsushima, 701-0192, Kurashiki, Japan.
Abstract:
Effects of conditioned emotional stimuli (CES), which induce psychological stress, on the expression of cerebral diazepam binding inhibitor (DBI) mRNA in mouse were examined using a communication box. Cerebral DBI mRNA expression significantly increased in a time-dependent manner after the application of CES. The maximal enhancement of DBI mRNA expression was observed 2 days after the application of CES, and this increase faded out over 7 days after the treatment. Flunitrazepam (1 mg/kg), an agonist for central benzodiazepine (BZD) receptors, completely abolished the CES-induced elevation of cerebral DBI contents and its mRNA expressions. These results indicate that cerebral DBI is enhanced by psychological stress, which is regulated by central BZD receptors.
