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Related Experiment Videos

Influence of polyhydroxysteroids on [Ca(2+)](i).

Irina G Agafonova1, Dmitry L Aminin, Larisa K Shubina

  • 1Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, Vladivostok, Russia. piboc@stl.ru

Steroids
|July 16, 2002
PubMed
Summary

Two marine steroids from Ophiopholis aculeata stimulate calcium influx in mouse cells without toxicity. These compounds may act as calcium agonists, enhancing cell membrane transport.

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Area of Science:

  • Marine biology
  • Pharmacology
  • Cell biology

Background:

  • Two biologically active disulfated polyhydroxysteroids from the Pacific brittle star Ophiopholis aculeata were previously shown to stimulate Ca(2+) influx.
  • The current study investigates the mechanisms and effects of these steroids on calcium signaling in various cell types.

Purpose of the Study:

  • To investigate the course and amplitude of calcium signals induced by O. aculeata steroids in different mouse cells.
  • To assess the cytotoxic and hemolytic effects of these steroids.
  • To explore the potential of these steroids as Ca(2+)-agonists.

Main Methods:

  • Utilized radio-isotope 45Ca(2+) and fluorescent calcium probes (quin-2/AM, fura-2/AM).
  • Employed spectrofluorimetry and microcytofluorimetry techniques.

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  • Tested effects on Ehrlich mouse carcinoma cells, mouse spleen lymphocytes, and mouse peritoneal macrophages.

    • Main Results:

    • Steroids induced a rapid, dose-dependent increase in cytosolic Ca(2+) in tested mouse cells (1-100 microg/ml).
    • No cytotoxic or hemolytic effects were observed within a wide concentration range.
    • L-type calcium channel blockers (verapamil, diltiazem, nifedipine) and EGTA inhibited the steroid-induced Ca(2+) influx.
    • Steroids stimulated human fibroblast proliferation and mouse macrophage lysosome activity.

    Conclusions:

    • The O. aculeata steroids act as Ca(2+)-agonists, promoting Ca(2+) transport across cell membranes.
    • These compounds exhibit potential therapeutic applications due to their cell-stimulating effects without apparent toxicity.