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Related Experiment Videos

A rat linkage map based on BC x LEW intercross.

A C M Bonné1, M G C W den Bieman, G F Gillissen

  • 1Department of Laboratory Animal Science, Faculty of Veterinary Medicine, Utrecht University, The Netherlands. Bonne@las.vet.uu.nl

Folia Biologica
|July 18, 2002
PubMed
Summary

Researchers developed a new genetic map using 258 markers in rats. This map covers 90% of the rat genome and aids in studying complex traits like cholesterol and copper metabolism.

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Area of Science:

  • Genetics
  • Genomics
  • Comparative Genomics

Background:

  • Genetic linkage maps are essential for understanding genome organization and identifying genes underlying complex traits.
  • Previous rat genetic maps have limitations in coverage and marker density.

Purpose of the Study:

  • To construct a high-density genetic linkage map for the rat genome.
  • To identify potential discrepancies with existing rat chromosome 7 maps.
  • To provide a tool for investigating the genetic basis of complex traits such as dietary cholesterol susceptibility and hepatic copper content.

Main Methods:

  • An F2 intercross population was generated between the BC/CpbU and LEW/OlaHsd inbred rat strains.
  • 258 polymorphic loci were genotyped to construct the genetic linkage map.

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  • The map's length, marker spacing, and genome coverage were calculated.

    • Main Results:

    • A genetic linkage map with 258 polymorphic loci was successfully constructed.
    • The map spans a sex-averaged genetic length of 1790 cM with an average marker spacing of 7.7 cM.
    • A discrepancy was noted for rat chromosome 7 compared to previously published maps.
    • The constructed map is estimated to cover approximately 90% of the rat genome.

    Conclusions:

    • The new genetic map provides comprehensive genome coverage and improved marker density for the rat.
    • This map serves as a valuable resource for quantitative trait loci (QTL) analysis and the study of complex genetic traits in rats.
    • The identified discrepancy in rat chromosome 7 warrants further investigation.