Blastoid transformation of lymphocytes in response to spermatozoa

Microlymphocyte transformation tests were done on 33 female rabbits immunized with different components of rabbit ejaculate. In rabbits injected with total ejaculate or washed spermatozoa a specific stimulation of lymphocytes could be demonstrated by exposure to washed spermatozoa. It is shown that it is necessary to notice the length of time for cultivation, the antigen concentration and especially the time-interval after the last immunization.