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Electrospray ionisation mass spectrometric detection of weak non-covalent interactions in nogalamycin-DNA complexes
Michelle L Colgrave1, Jennifer L Beck, Margaret M Sheil
1Department of Chemistry, University of Wollongong, NSW, 2522, Australia.
Chemical Communications (Cambridge, England)
|July 18, 2002
Abstract:
We demonstrate the use of electrospray ionisation mass spectrometry (ESI-MS) in high salt solutions for the analysis of weak non-covalent complexes of the anthracycline antibiotic nogalamycin with novel DNA hairpin structures; high signal-to-noise ratios for the complexes in the absence of bound Na+ ions permits relative binding affinities to be estimated.