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Tolerization as a tool for generating novel monoclonal antibodies
Felicity L Holbrook1, Ian C Nicholson, Heddy Zola
1Department of Paediatrics and CRC for Diagnostics, Child Health Research Institute, Adelaide University, Adelaide, South Australia, Australia.
Immunology and Cell Biology
|July 18, 2002
Summary
Inducing tolerance to common antigens allows for the creation of highly specific monoclonal antibodies. This review explores strategies for generating these valuable reagents by using protein mixtures or purified proteins as tolerogens.
Area of Science:
- Immunology
- Biotechnology
Background:
- Hybridoma technology is a cornerstone for producing monoclonal antibodies.
- Current demands necessitate antibodies with higher specificity, particularly for distinguishing closely related antigens.
Purpose of the Study:
- To review strategies for generating high-specificity monoclonal antibodies.
- To detail methods for overcoming limitations in distinguishing closely related antigens.
Main Methods:
- Utilizing induction of tolerance towards common antigens.
- Employing complex protein mixtures or purified proteins as tolerogens.
- Subsequent immunization with a closely related immunogen.
Main Results:
- Successful generation of elusive high-specificity reagents.
- Enabling the recovery of antibodies that can distinguish between closely related antigens.
Conclusions:
- Tolerance induction is a viable strategy for producing advanced monoclonal antibodies.
- These methods expand the utility of hybridoma technology for complex immunological research.