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Related Experiment Videos

Do mossy fibers release GABA?

Matthew C Walker1, Arnaud Ruiz, Dimitri M Kullmann

  • 1Department of Clinical and Experimental Epilepsy, Institute of Neurology, Queen Square, London WC1N 3BG, UK. m.walker@ion.ucl.ac.uk

Epilepsia
|July 18, 2002
PubMed
Summary

This study provides evidence for a gamma-aminobutyric acid (GABA) signal released by mossy fibers in the hippocampus. This inhibitory signal may play a role in development and is upregulated after seizures.

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Area of Science:

  • Neuroscience
  • Neurophysiology

Background:

  • Mossy fibers are the primary excitatory projection from dentate gyrus granule cells to the hippocampus.
  • They are implicated in epileptogenesis and seizure generation.
  • Mossy fibers release multiple neurotransmitters including glutamate, adenosine, dynorphin, zinc, and GABA.

Purpose of the Study:

  • To present physiologic evidence of gamma-aminobutyric acid (GABA) release by mossy fibers.
  • To investigate the modulation of this GABAergic signal by epileptic activity.

Main Methods:

  • Whole-cell voltage clamp recordings were performed on CA3 pyramidal cells in guinea pig hippocampal slices.
  • Stimulating electrodes were placed in the stratum granulosum to recruit mossy fiber projections to CA3.

Main Results:

  • Electrical stimulation recruiting dentate granule cells elicited monosynaptic GABAA receptor-mediated signals in CA3 pyramidal neurons.
  • These inhibitory signals exhibited characteristics of mossy fiber-CA3 synapses, including metabotropic glutamate receptor sensitivity, facilitation, and NMDA receptor independence.

Conclusions:

  • Compelling physiologic evidence supports the existence of a mossy fiber GABAergic signal.
  • The precise role of this signal is unclear but may be developmentally important.
  • Evidence suggests this GABAergic signal is transiently upregulated post-seizure, potentially exerting inhibitory or disinhibitory effects requiring further investigation.

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