Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Constructing plant radiation hybrid panels.

Julie Wardrop1, John Snape, Wayne Powell

  • 1Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK.

The Plant Journal : for Cell and Molecular Biology
|July 18, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From photoperiod thresholds to photoperiod sensitivity: dual strategies for cost-effective speed breeding and climate-ready barley.

Frontiers in plant science·2026
Same author

On the origin of the late-flowering ppd-H1 allele in barley.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2025
Same author

Integrating molecular genetics with plant breeding to deliver impact.

Plant physiology·2025
Same author

Investigating the genetic control of plant development in spring barley under speed breeding conditions.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2024
Same author

Tin from Uluburun shipwreck shows small-scale commodity exchange fueled continental tin supply across Late Bronze Age Eurasia.

Science advances·2022
Same author

Analysis of historical selection in winter wheat.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2022

Radiation hybrid (RH) mapping uses X-ray induced chromosome breakage for physical mapping. This study details generating plant radiation hybrids for mapping large crop genomes.

Area of Science:

  • Genetics
  • Genomics
  • Molecular Biology

Background:

  • Radiation hybrid (RH) mapping is a somatic cell genetic technique for constructing long-range physical maps of chromosomes.
  • This statistical method uses X-ray induced breakage to determine marker distance and order on chromosomes, applicable to single chromosomes or whole genomes.

Purpose of the Study:

  • To describe the generation of plant (barley) radiation hybrids and their in vitro culture.
  • To demonstrate the utility of PCR-based markers for verifying hybrid status and genome coverage.

Main Methods:

  • Generation of plant radiation hybrids.
  • In vitro culture of radiation hybrids.
  • PCR-based marker systems for verification and genome coverage assessment.

Related Experiment Videos

Main Results:

  • Successful generation of plant radiation hybrids.
  • Verification of hybrid status and demonstration of genome coverage using PCR markers.
  • Established RH panels suitable for physical mapping and related applications.

Conclusions:

  • Radiation hybrid panels are valuable resources for physical mapping, map-based cloning, and sequence contig assembly.
  • RH resources are particularly beneficial for the physical characterization of crop plants with large genomes.