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Interaction of Porphyromonas gingivalis with KB cells: comparison of different clinical isolates
1Department of Medical Microbiology, University Hospital of Jena, Jena, Germany.
Abstract:
The ability of different Porphyromonas gingivalis strains (15 clinical isolates and ATCC 33277) to attach to and invade KB cells, in relation to other properties such as release of interleukin (IL)-6 and IL-8, cytotoxicity, proteolytic activity and types of fimbriae genes present, was examined. A hierarchical cluster analysis based on adherence and internalization resulted in four groups. Eight of the 15 clinical isolates belonged to a cluster group whose adherence and internalization were about 10% those of the ATCC strain. A negative correlation between lysine-specific protease activity and adherence was found. In all cases the released concentrations of IL-6 and IL-8 were very low. Only one strain was found to be cytotoxic to KB cells. Principal components analysis demonstrated correlations between adherence, internalization and autoaggregation. Most strains had fimA type I and II, type I being associated with elastase-like activity. The ability of P. gingivalis to invade epithelial cells may be a key factor for maintaining periodontal disease.
Insights
Porphyromonas gingivalis strains vary in their ability to invade epithelial cells, a key factor in periodontal disease. Researchers found differences in adherence, internalization, and protease activity among clinical isolates.
Area of Science:
- Microbiology
- Oral Health
- Bacterial Pathogenesis
Background:
- Porphyromonas gingivalis is a key pathogen in periodontal disease.
- Understanding bacterial virulence factors is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the adherence and invasion capabilities of Porphyromonas gingivalis strains.
- To correlate these abilities with other virulence factors like protease activity and fimbriae gene expression.
Main Methods:
- Utilized hierarchical cluster analysis and principal components analysis.
- Examined adherence, internalization, interleukin (IL)-6 and IL-8 release, cytotoxicity, proteolytic activity, and fimbriae genes.
- Tested 15 clinical isolates and the ATCC 33277 strain against KB cells.
Main Results:
- Hierarchical clustering revealed four distinct groups based on adherence and internalization.
- Eight clinical isolates showed significantly lower adherence and internalization compared to the ATCC strain.
- A negative correlation was observed between lysine-specific protease activity and adherence.
- Most strains possessed fimA types I and II, with type I linked to elastase-like activity.
Conclusions:
- Porphyromonas gingivalis invasion of epithelial cells is a critical factor in periodontal disease pathogenesis.
- Strain variability in virulence factors influences disease progression.
- Further research into these factors could lead to targeted therapies for periodontal disease.