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Immobilization of invertase on rice husk using polyethylenimine
1Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India. sfdsouza@apsara.barc.ernet.in
Journal of Biochemical and Biophysical Methods
|July 18, 2002
Abstract:
Washed and dried rice husk was coated with 2% polyethylenimine (PEI). Invertase was immobilized on this support through adsorption followed by cross-linking with 2% glutaraldehyde. Immobilized enzyme was reused for the hydrolysis of sucrose without loss in activity. This approach may serve as a simple technique in the future for the covalent immobilization of enzymes on lignocellulosic supports.