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Rapid detection of a molecular marker for chloroquine-resistant falciparum malaria
Rémy Durand1, Virginie Huart, Sayeh Jafari
1Centre National de Référence pour la Chimiosensibilité du Paludisme, Assistance Publique-Hôpitaux de Paris, Laboratoire de Parasitologie, Hôpital Bichat-Claude Bernard, 46 Rue Henri Huchard, 75877 Paris Cedex 18, France. remy.durand@bch.ap-hop-paris.fr
Antimicrobial Agents and Chemotherapy
|July 18, 2002
Abstract:
A PCR-based technique using molecular beacons was developed to detect the chloroquine resistance-associated pfcrt K76T point mutation in Plasmodium falciparum. One hundred thirty African clinical isolates were tested by the new method in comparison with the PCR-restriction fragment length polymorphism method. This rapid and inexpensive genomic assay could expand the possibilities for monitoring chloroquine resistance.