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Published on: September 18, 2013

Inhibition of prostate tumor angiogenesis by the tumor suppressor CEACAM1

Olga Volpert1, Weiping Luo, Ta-Jen Liu

  • 1Department of Microbiology-Immunology, R. H. Lurie Cancer Center, Northwestern University Medical School, Chicago, Illinois 60611, USA.

The Journal of Biological Chemistry
|July 18, 2002
PubMed

Insights

Carcinoma cell adhesion molecule CEACAM1 suppresses tumor growth by inhibiting angiogenesis. CEACAM1 expression in prostate cancer cells promotes the secretion of factors that block blood vessel formation and induce cancer cell death.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Medicine

Background:

  • Carcinoma cell adhesion molecule CEACAM1 previously identified as a tumor suppressor in prostate cancer.
  • CEACAM1 expression inhibits prostate cancer cell growth in vivo but not in vitro, suggesting a role in anti-angiogenesis.

Purpose of the Study:

  • To investigate the mechanism by which CEACAM1 suppresses tumor growth, specifically its effect on angiogenesis.
  • To determine if CEACAM1 induces the production of anti-angiogenic factors.

Main Methods:

  • Expression of CEACAM1 in DU145 prostate cancer cells.
  • Analysis of conditioned medium from CEACAM1-expressing cells for effects on endothelial and epithelial cells in vitro.
  • Assessment of conditioned medium's effect on corneal neovascularization in vivo.
  • Evaluation of endothelial cell apoptosis induction by conditioned medium.

Main Results:

  • Conditioned medium from CEACAM1-expressing cells inhibited endothelial cell growth, migration, and induced apoptosis.
  • CEACAM1 expression blocked endothelial cell migration towards vascular endothelial growth factor.
  • CEACAM1 inhibited basic fibroblast growth factor-induced corneal neovascularization in vivo.
  • Only CEACAM1 mutants suppressing tumor growth in vivo induced endothelial cell apoptosis.

Conclusions:

  • CEACAM1-mediated tumor suppression in prostate carcinoma is partly due to the inhibition of tumor angiogenesis.
  • CEACAM1 promotes the secretion of factors that specifically target endothelial cells, inhibiting neovascularization and inducing apoptosis.

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