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Double Whole Mount in situ Hybridization of Early Chick Embryos
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Double Whole Mount in situ Hybridization of Early Chick Embryos

Published on: October 26, 2008

An improved method for whole-mount in situ hybridization of Heterodera schachtii juveniles

Bartel Vanholme1, Jan De Meutter, Tom Tytgat

  • 1Vakgroep Moleculaire Biotechnologie, Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen, Universiteit Gent, Coupure links 653, 9000 Gent, Belgium.

Parasitology Research
|July 18, 2002
PubMed

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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...