Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorinated NAD as an affinity surfactant.

Janice L Panza1, Alan J Russell, Eric J Beckman

  • 1Benesch Friendlander Coplan & Aronoff, Cleveland, OH, USA. jpanza@bfca.com

Chemical Communications (Cambridge, England)
|July 19, 2002
PubMed
Summary

Nicotinamide adenine dinucleotide (NAD) with a perfluoropolyether tail functions as a surfactant. This enables the extraction of horse liver alcohol dehydrogenase (HLADH) into a fluorous solvent.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human Disposition, Metabolism, and Excretion of Sevasemten (EDG-5506), a Selective Modulator of Fast Myosin in Healthy Volunteers.

Journal of clinical pharmacology·2026
Same author

Effects of sevasemten (EDG-5506) on safety, biomarkers, and functional measures in adults with Becker muscular dystrophy: results of a phase 1b, open-label study.

EBioMedicine·2026
Same author

Exploring the evidence for the use of protein biomarkers of muscular damage and disease progression in Duchenne and Becker muscular dystrophy: a systematic review and meta-analysis protocol.

BMJ open·2025
Same author

Short-term daily deflazacort decreases membrane permeability and increases maximum force in <i>mdx</i> mice.

American journal of physiology. Cell physiology·2025
Same author

Heterozygous <i>MYH7</i> R403Q mutation impairs left atrial mitochondrial function in a Yucatan mini-pig model of genetic nonobstructive hypertrophic cardiomyopathy.

Journal of applied physiology (Bethesda, Md. : 1985)·2025
Same author

A Phase 1, Double-Blind, Placebo-Controlled Trial of Sevasemten (EDG-5506), a Selective Modulator of Fast Skeletal Muscle Contraction, in Healthy Volunteers and Adults With Becker Muscular Dystrophy.

Muscle & nerve·2025

Area of Science:

  • Biochemistry
  • Separation Science

Background:

  • Enzyme extraction and purification are crucial in biochemical research and industrial applications.
  • Traditional methods often face challenges in efficiency and selectivity.
  • Surfactants play a role in modifying interfacial properties for separation processes.

Purpose of the Study:

  • To investigate the efficacy of a novel nicotinamide adenine dinucleotide (NAD) derivative as an affinity surfactant.
  • To demonstrate the extraction of horse liver alcohol dehydrogenase (HLADH) using this specialized surfactant.
  • To explore the application of fluorous phase separation for enzyme recovery.

Main Methods:

  • Synthesis of a NAD derivative functionalized with a perfluoropolyether tail.
  • Utilizing the NAD-perfluoropolyether conjugate as an affinity surfactant.
  • Performing liquid-liquid extraction from an aqueous medium into a fluorous solvent.
  • Assessing the recovery and activity of horse liver alcohol dehydrogenase (HLADH).

Main Results:

  • The NAD-perfluoropolyether conjugate effectively acted as an affinity surfactant.
  • Successful extraction of HLADH from an aqueous phase into a fluorous solvent was achieved.
  • The surfactant facilitated the partitioning of the target enzyme into the fluorous phase.

Conclusions:

  • Nicotinamide adenine dinucleotide (NAD) derivatives with fluorous tails can serve as effective affinity surfactants.
  • This approach offers a novel method for enzyme extraction and purification.
  • Fluorous phase separation presents a viable strategy for isolating biomolecules like HLADH.

Related Experiment Videos