Related Experiment Videos
Preimplantation gender diagnosis by fluorescence in situ hybridization
Yanwen Xu1, Guanglun Zhuang, Yimin Shu
1Reproductive Research Center, Department of Obstetrics and Gynecology, The First Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou 510080, China. xuyanwen@public.guangzhou.gd.cn
Objective:
To describe the clinical application of fluorescence in situ hybridization (FISH ) in preimplantation gender diagnosis.
Methods:
Preimplantation gender diagnosis was performed in 2 female hemophilia A carriers, 1 male patient with glucose-6-phosphate dehydrogenase (G-6-PD) deficiency and 2 male patients with Y chromosome abnormality. Embryo sex was identified by FISH in total of 6 treatment cycles.
Results:
A total of 123 cumulus-oocytes were retrieved in 6 treatment cycles. Sixty-one embryos were available for embryo biopsy. The success rate of biopsy was 86.9% (53/61), with a further cleavage rate of 62.3% (33/53). In the FISH procedure, one cell was lost during fixation, leading to a 98.1% (52/53) fixation rate. Totally, 16 female embryos and 3 male embryos were transferred to 5 patients in 6 cycles. Three healthy babies were born. The diagnosis was confirmed by subsequent analysis of amniocytes and embryonic buds after embryo reduction.
Conclusions:
FISH is an efficient and reliable technique for determining the sex of human preimplantation embryos. Selective abortion and births of affected children can be avoided by preimplantation gender diagnosis.