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Published on: December 21, 2011
Factor Xa induces mitogenesis of coronary artery smooth muscle cell via activation of PAR-2
Bon Hun Koo1, Kwang Hoe Chung, Ki Chul Hwang
1Department of Biochemistry, College of Science, and Bioproducts Research Center, Yonsei University, 120-749, Seoul, South Korea.
Abstract:
Factor Xa-induced stimulation of coronary artery smooth muscle cells (CASMC) was investigated by analyzing [(3)H]thymidine incorporation, cell proliferation, and ERK-1/2 activation. Exposure of the cells to factor Xa evoked a time-dependent activation of ERK-1/2 with increased [(3)H]thymidine incorporation and cell proliferation. The factor Xa-induced ERK-1/2 activation was not desensitized by preincubation of the cells with thrombin. However, ERK-1/2 activation was markedly attenuated by prior exposure of the cells to protease-activated receptor-2 (PAR-2) activating peptide, SLIGKV. The mitogenic effect of factor Xa was significantly reduced in the presence of anti-PAR-2 monoclonal antibody. Several lines of experimental evidence indicate that factor Xa-induced mitogenesis of CASMC is a cellular process mediated by PAR-2 activation.
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