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Establishing two-dimensional gels for the analysis of Leishmania proteomes
Nathalie Acestor1, Slavica Masina, John Walker
1Institute of Biochemistry, University of Lausanne, Switzerland.
Proteomics
|July 19, 2002
Abstract:
Several different sample preparation methods for two-dimensional electrophoresis (2-DE) analysis of Leishmania parasites were compared. From this work, we were able to identify a solubilization method using Nonidet P-40 as detergent, which was simple to follow, and which produced 2-DE gels of high resolution and reproducibility.