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Related Experiment Video

Updated: Jul 19, 2026

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer
07:13

Initiation of Metastatic Breast Carcinoma by Targeting of the Ductal Epithelium with Adenovirus-Cre: A Novel Transgenic Mouse Model of Breast Cancer

Published on: March 26, 2014

Targeting mammary epithelial cells using a bacterial artificial chromosome.

Tim M Wintermantel1, Anja K Mayer, Günther Schütz

  • 1Division Molecular Biology of the Cell I, Deutsches Krebsforschungszentrum, Heidelberg, Germany.

Genesis (New York, N.Y. : 2000)
|July 19, 2002
PubMed
Summary

We developed transgenic mice with Cre recombinase specifically in mammary gland epithelial cells using the whey acidic protein (WAP) gene. These WAP-iCre mice enable efficient genetic recombination in the mammary gland during lactation.

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In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection
05:36

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

Published on: February 10, 2023

Area of Science:

  • * Molecular Biology
  • * Genetics
  • * Developmental Biology

Background:

  • * Mammary gland development and function are complex processes requiring precise genetic regulation.
  • * Tools for targeted genetic manipulation in specific cell types are crucial for studying these processes.
  • * Existing methods may lack specificity or efficiency in mammary epithelial cells.

Purpose of the Study:

  • * To generate transgenic mouse lines for efficient Cre recombinase expression in mammary epithelial cells.
  • * To utilize the whey acidic protein (WAP) gene promoter for cell-specific expression.
  • * To establish a reliable system for studying mammary gland biology through targeted recombination.

Main Methods:

  • * Construction of a bacterial artificial chromosome (PAC) containing the WAP gene and an improved Cre recombinase (iCre) coding sequence.
  • * Modification of the PAC using homologous recombination in E. coli.
  • * Generation and characterization of transgenic mouse lines (WAPiCre).

Main Results:

  • * Three out of four generated transgenic lines exhibited high-efficiency Cre recombinase expression.
  • * Expression was targeted to the majority of mammary epithelial cells during lactation.
  • * Efficient recombination of LoxP-flanked DNA sequences was observed in nearly all epithelial cells by lactation day 3.

Conclusions:

  • * The WAPiCre transgenic mouse lines provide a powerful tool for studying mammary gland biology.
  • * These mice allow for efficient, lactation-specific, and cell-type-specific genetic manipulation.
  • * This system facilitates research into gene function and developmental processes within the mammary epithelium.