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Targeting mammary epithelial cells using a bacterial artificial chromosome.

Tim M Wintermantel1, Anja K Mayer, Günther Schütz

  • 1Division Molecular Biology of the Cell I, Deutsches Krebsforschungszentrum, Heidelberg, Germany.

Genesis (New York, N.Y. : 2000)
|July 19, 2002
PubMed
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We developed transgenic mice with Cre recombinase specifically in mammary gland epithelial cells using the whey acidic protein (WAP) gene. These WAP-iCre mice enable efficient genetic recombination in the mammary gland during lactation.

Area of Science:

  • * Molecular Biology
  • * Genetics
  • * Developmental Biology

Background:

  • * Mammary gland development and function are complex processes requiring precise genetic regulation.
  • * Tools for targeted genetic manipulation in specific cell types are crucial for studying these processes.
  • * Existing methods may lack specificity or efficiency in mammary epithelial cells.

Purpose of the Study:

  • * To generate transgenic mouse lines for efficient Cre recombinase expression in mammary epithelial cells.
  • * To utilize the whey acidic protein (WAP) gene promoter for cell-specific expression.
  • * To establish a reliable system for studying mammary gland biology through targeted recombination.

Main Methods:

  • * Construction of a bacterial artificial chromosome (PAC) containing the WAP gene and an improved Cre recombinase (iCre) coding sequence.

Related Experiment Videos

  • * Modification of the PAC using homologous recombination in E. coli.
  • * Generation and characterization of transgenic mouse lines (WAPiCre).

    • Main Results:

    • * Three out of four generated transgenic lines exhibited high-efficiency Cre recombinase expression.
    • * Expression was targeted to the majority of mammary epithelial cells during lactation.
    • * Efficient recombination of LoxP-flanked DNA sequences was observed in nearly all epithelial cells by lactation day 3.

    Conclusions:

    • * The WAPiCre transgenic mouse lines provide a powerful tool for studying mammary gland biology.
    • * These mice allow for efficient, lactation-specific, and cell-type-specific genetic manipulation.
    • * This system facilitates research into gene function and developmental processes within the mammary epithelium.