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[Two-step purification of estradiol calf uterine cytosol receptor]
Abstract:
The use of two affinity chromatography methods: heparin-Ultrogel and estradiol 7 alpha-(CH2)3-poly (alanine-lysine)-agarose, allows us to purify the calf uterine estradiol cytosol receptor with a 20% recovery. Electrophoresis under denaturing conditions allows us to estimate its molecular weight as 70 000 dalton.
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