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Updated: Sep 30, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
Published on: March 14, 2013
High-throughput liquid chromatography ultraviolet/mass spectrometric analysis of combinatorial libraries using an
Liling Fang1, Jason Cournoyer, Michael Demee
1ChemRx Division, Discovery Partners International, 385 Oyster Point Blvd. Suite #1, South San Francisco, CA 94080, USA. fang@chemrx.com
Abstract:
We report a high-throughput liquid chromatography/mass spectrometry (LC/MS) protocol for analyzing large combinatorial libraries using an eight-channel parallel LC/UV/MS (MUX-LCT) system. System configuration, linear response range in UV absorbance, LC column selection, and flow rate were optimized for 24 h/7 day unattended operations. Combinatorial libraries were analyzed on this system at a rate of 3200 compounds per day for a 3.5 min cycle time per injection. This parallel system is compared with a single-channel system in terms of performance and operation.
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