Flow cytometric determination of lipid peroxidation using fluoresceinated phosphoethanolamine

In conclusion, we describe the use of fluor-DHPE as a flow cytometric probe to assess lipid peroxidation in erythrocytes. The stability and nonexchangeability of this probe make it suitable for monitoring lipid peroxidation in a particular cell type via flow cytometry, which can be done in the presence of other cells. Application of the probe in erythrocytes from rats fed a vitamin E-deficient diet demonstrated a higher susceptibility to lipid peroxidation among these cells than among erythrocytes from rats fed a normal diet. The current flow cytometric lipid peroxidation detection method can be interfaced directly with several standard techniques that are available to measure specific blood cell populations via flow cytometry.