Interleukin-5 messenger RNA expression in peripheral blood mononuclear cells from patients with bronchial asthma and eosinophilia
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Updated: Sep 30, 2026
Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation
Published on: May 30, 2020
Takao Ishizuka1, Tamotsu Ishizuka, Shigenori Iwamae
1First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Abstract:
Interleukin-5 (IL-5) plays an important role in allergic diseases accompanied by eosinophilia. We examined IL-5 messenger RNA (mRNA) expression in peripheral blood mononuclear cells (PBMC) isolated from 13 patients with bronchial asthma, 1 patient with chronic eosinophilic pneumonia, 2 patients with idiopathic eosinophilia, and 5 control subjects. IL-5 mRNA was expressed in PBMC from 2 of 13 patients with asthma, 1 patient with chronic eosinophilic pneumonia, and 1 of 2 patients with idiopathic eosinophilia. IL-5 mRNA expression was not detected in PBMC from control subjects. PBMC from a patient with chronic eosinophilic pneumonia expressed IL-5 mRNA spontaneously. Prednisolone treatment decreased his clinical symptoms and IL-5 mRNA expression. IL-5 mRNA expression preceded revival of the disease. These observations show that IL-5 plays a role in the condition, accompanied by eosinophilia, and IL-5 mRNa examination in PBMC by means of reverse transcription-polymerase chain reaction may be useful to predict the activity of eosinophilia.
Insights
Interleukin-5 (IL-5) messenger RNA (mRNA) is elevated in patients with eosinophilic disorders like asthma. Measuring IL-5 mRNA in blood cells may help predict eosinophilia activity.
Area of Science:
- Immunology
- Molecular Biology
Background:
- Interleukin-5 (IL-5) is implicated in allergic diseases associated with eosinophilia.
- Eosinophilia is a condition characterized by elevated levels of eosinophils, a type of white blood cell.
Purpose of the Study:
- To investigate the expression of Interleukin-5 (IL-5) messenger RNA (mRNA) in peripheral blood mononuclear cells (PBMC) of patients with eosinophilic conditions.
- To assess the potential of IL-5 mRNA detection as a biomarker for eosinophilia activity.
Main Methods:
- Peripheral blood mononuclear cells (PBMC) were isolated from patients with bronchial asthma, chronic eosinophilic pneumonia, idiopathic eosinophilia, and healthy controls.
- Interleukin-5 (IL-5) messenger RNA (mRNA) expression was analyzed using reverse transcription-polymerase chain reaction (RT-PCR).
Main Results:
- IL-5 mRNA was detected in PBMC from patients with asthma, chronic eosinophilic pneumonia, and idiopathic eosinophilia, but not in controls.
- A patient with chronic eosinophilic pneumonia showed spontaneous IL-5 mRNA expression, which decreased with prednisolone treatment.
- IL-5 mRNA levels correlated with disease activity and preceded symptom recurrence.
Conclusions:
- Interleukin-5 (IL-5) plays a significant role in conditions characterized by eosinophilia.
- Detection of IL-5 mRNA in PBMC via RT-PCR may serve as a valuable tool for predicting eosinophilia activity.
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