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Updated: Sep 30, 2026
Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation
Published on: May 30, 2020
Interleukin-5 messenger RNA expression in peripheral blood mononuclear cells from patients with bronchial asthma and
Takao Ishizuka1, Tamotsu Ishizuka, Shigenori Iwamae
1First Department of Internal Medicine, Gunma University School of Medicine, Maebashi, Japan.
Abstract:
Interleukin-5 (IL-5) plays an important role in allergic diseases accompanied by eosinophilia. We examined IL-5 messenger RNA (mRNA) expression in peripheral blood mononuclear cells (PBMC) isolated from 13 patients with bronchial asthma, 1 patient with chronic eosinophilic pneumonia, 2 patients with idiopathic eosinophilia, and 5 control subjects. IL-5 mRNA was expressed in PBMC from 2 of 13 patients with asthma, 1 patient with chronic eosinophilic pneumonia, and 1 of 2 patients with idiopathic eosinophilia. IL-5 mRNA expression was not detected in PBMC from control subjects. PBMC from a patient with chronic eosinophilic pneumonia expressed IL-5 mRNA spontaneously. Prednisolone treatment decreased his clinical symptoms and IL-5 mRNA expression. IL-5 mRNA expression preceded revival of the disease. These observations show that IL-5 plays a role in the condition, accompanied by eosinophilia, and IL-5 mRNa examination in PBMC by means of reverse transcription-polymerase chain reaction may be useful to predict the activity of eosinophilia.
Insights
Interleukin-5 (IL-5) messenger RNA (mRNA) is elevated in patients with eosinophilic disorders like asthma. Measuring IL-5 mRNA in blood cells may help predict eosinophilia activity.
Area of Science:
- Immunology
- Molecular Biology
Background:
- Interleukin-5 (IL-5) is implicated in allergic diseases associated with eosinophilia.
- Eosinophilia is a condition characterized by elevated levels of eosinophils, a type of white blood cell.
Purpose of the Study:
- To investigate the expression of Interleukin-5 (IL-5) messenger RNA (mRNA) in peripheral blood mononuclear cells (PBMC) of patients with eosinophilic conditions.
- To assess the potential of IL-5 mRNA detection as a biomarker for eosinophilia activity.
Main Methods:
- Peripheral blood mononuclear cells (PBMC) were isolated from patients with bronchial asthma, chronic eosinophilic pneumonia, idiopathic eosinophilia, and healthy controls.
- Interleukin-5 (IL-5) messenger RNA (mRNA) expression was analyzed using reverse transcription-polymerase chain reaction (RT-PCR).
Main Results:
- IL-5 mRNA was detected in PBMC from patients with asthma, chronic eosinophilic pneumonia, and idiopathic eosinophilia, but not in controls.
- A patient with chronic eosinophilic pneumonia showed spontaneous IL-5 mRNA expression, which decreased with prednisolone treatment.
- IL-5 mRNA levels correlated with disease activity and preceded symptom recurrence.
Conclusions:
- Interleukin-5 (IL-5) plays a significant role in conditions characterized by eosinophilia.
- Detection of IL-5 mRNA in PBMC via RT-PCR may serve as a valuable tool for predicting eosinophilia activity.
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