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Updated: Sep 30, 2026
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Published on: December 6, 2024
125I-iomazenil-benzodiazepine receptor binding during psychological stress in rats
Nobuyoshi Fukumitsu1, Daisuke Tsuchida, Shigeyuki Ogi
1Department of Radiology, Jikei University School of Medicine, Tokyo, Japan. GZL13162@nifty.ne.jp
Objective:
We investigated the changes in 125I-iomazenil (125I-IMZ) benzodiazepine receptor (BZR) binding with psychological stress in a rat model.
Methods:
Six male Wistar rats were placed under psychological stress for 1 hour by using a communication box. No physical stress was not received. 1.85 MBq of 125I-IMZ was injected into the lateral tail vein and the rat was killed 3 hours later. Twenty-micrometer-thick sections of the brain were collected and % injected dose per body weight (%ID/BW) of eleven regions (frontal, parietal, temporal, occipital cortices, caudate putamen, accumubens nuclei, globus pallidus, amygdala, thalamus, hippocampus and hypothalamus) were calculated by autoradiography. The %ID/BW of rats which were placed under psychological stress was compared with that of 6 control rats.
Results:
The %ID/BW of rats which were placed under psychological stress diffusely tended to show a reduction in 125I-IMZ-BZR binding. A significant decrease in BZR binding was observed in the hippocampus of the rats which were placed under psychological stress.
Conclusion:
125I-IMZ-BZR binding tended to decrease throughout the brain.
