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Chromatographic reactors based on biological activity.

Ales Podgornik1, Tatiana B Tennikova

  • 1BIA Separations doo, Ljubljana, Slovenia. Ales.Podgornik@guest.arnes.si

Advances in Biochemical Engineering/Biotechnology
|July 20, 2002
PubMed
Summary

Enzyme catalyzed reactions in chromatographic bioreactors offer advantages like separation during reaction and overcoming equilibrium limits. This review covers these systems, enzyme immobilization, and advanced chromatographic supports.

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Area of Science:

  • Biocatalysis and Chemical Engineering
  • Separation Science

Background:

  • Enzyme catalyzed reactions are increasingly studied in chromatographic reactors.
  • These systems offer unique advantages, including in-situ separation and overcoming thermodynamic equilibrium limitations.
  • The removal of reaction products or inhibitors is also facilitated.

Purpose of the Study:

  • To provide an overview of chromatographic bioreactor systems.
  • To discuss enzyme immobilization techniques for enhanced activity.
  • To highlight modern chromatographic supports with improved hydrodynamic properties.

Main Methods:

  • Review of existing literature on chromatographic bioreactors.
  • Discussion of enzyme immobilization strategies.
  • Analysis of advanced chromatographic support materials.

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Main Results:

  • Chromatographic bioreactors enable reactions to proceed beyond thermodynamic equilibrium.
  • Enzyme immobilization and advanced supports enhance reactor performance.
  • In-situ separation is a key advantage of these systems.

Conclusions:

  • Chromatographic bioreactors represent a promising approach for efficient biocatalysis.
  • Further development in enzyme immobilization and support materials will enhance their applicability.
  • These systems offer significant potential for driving reactions and managing inhibitors.