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[Differentially expressed genes in vascular endothelial cell line ECV 304 induced by high density lipoprotein]
Wencheng Zhang1, Baosheng Chen, Wuwei Zeng
1National Laboratory of Medical Molecular Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China.
Objective:
To isolate and clone the differentially expressed genes in vascular endothelial cell induced by high density lipoprotein (HDL) so as to study the anti- atherogenesis molecular mechanism of HDL.
Methods:
Differential display reverse transcription PCR method was used to analyze the differentially expressed cDNA in vascular endothelial cell line ECV 304 induced by HDL. After sequencing and homology research, several differentially expressed cDNA fragments were confirmed by Northern blot analysis.
Results:
Up-regulated and down-regulated cDNA fragments in ECV 304 induced by HDL were isolated. Nine cDNA fragments were highly homologous to the known human genes and four were fragments of novel genes. The known up-regulated genes included genes of human STE20-like kinase, PBK1 protein, transglutaminase, myosin alkali light chain, apobec-1 binding protein 1, and death- associated protein (DAP). The known down-regulated genes included genes of ribosomal protein L7a (RPL7A), voltage-dependent anion channel isoform 2, and glycinamide ribonucleotide transformylase. Northern blot analysis revealed that the expression levels of transglutaminase and apobec-1 binding protein 1 were upregulated by 50% and 70% respectively.
Conclusion:
HDL upregulates the expression of apobec-1 binding protein 1 and transglutaminase in endothelial cells. The high level expression of transglutaminase and apobec-1 binding protein 1 in endothelial cells induced by HDL may be related to anti-atherogenesis function of HDL.