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Updated: Sep 30, 2026
Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
Published on: March 7, 2022
[Effect of superoxide dismutase on adhesion molecules expression in skeletal muscle ischemia/reperfusion injury in
Songcen Lu1, Xintao Wang, Lichen Wen
1Department of Hand Surgery,The Second Affiliated Hospital Of Harbin Medical University, Harbin 150086 China.
Objective:
To study the effect of SOD on the expression of CD11b/CD18 and ICAM-1 after skeletal muscle ischemia / reperfusion injury in rats.
Methods:
Establish the rat model of hind limb ischemia / reperfusion. One hundred and two rats were divided into 5 groups: sham operation group (Group I n = 6), ischemia group (GroupII n = 6), ischemia / reperfusion injury group (Group III n = 30), normal saline treatment group (Group IV n = 30), superoxide dismutase treatment group (Group V n = 30). Flow cytometric analysis and immunohistochemistry were used to assess the expression of CD11b/CD18 on the leukocytes, ICAM-1 in skeletal muscle at the end of ischemia and 1h, 2h, 4h,8h,12h after reperfusion. The changes of MDA in the plasma,MPO in the skeletal muscle and the tissue morphology were studied too.
Results:
In group III,the expression of CD11b/CD18, ICAM-1,MDA,MPO were increased evidently. The longer time of reperfusion,the more serious the skeletal muscle's injury was. In group V, the changes were ameliorated as compared with group III (P < 0.05), group IV has no difference with group III.
Conclusion:
These data indicate that the expression of CD11b/CD18 and ICAM-1 were significantly correlated with skeletal muscle ischemia/reperfusion injury. SOD can inhibit the expression of free radicals and the adhesion molecules,consequently skeletal muscle ischemia/reperfusion injury is prevented.
