Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dynamic changes in cell wall polysaccharides during wheat seedling development.

Nicolai Obel1, Andrea Celia Porchia, Henrik Vibe Scheller

  • 1Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark.

Phytochemistry
|July 20, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ectopic expression of pectate lyase <i>Ptxt</i>PL1-27 in aspen affects leaf cuticle development.

iScience·2025
Same author

Cell wall β-1,4-galactan regulated by the BPC1/BPC2-GALS1 module aggravates salt sensitivity in Arabidopsis thaliana.

Molecular plant·2020
Same author

Editorial: Proceedings of ICPSBBB 2018 - 2<sup>nd</sup> International Conference on Plant Synthetic Biology, Bioengineering and Biotechnology.

Frontiers in plant science·2020
Same author

No evidence for transient transformation via pollen magnetofection in several monocot species.

Nature plants·2020
Same author

Synthesis and Function of Complex Sphingolipid Glycosylation.

Trends in plant science·2020
Same author

Molecular characteristics of plant UDP-arabinopyranose mutases.

Glycobiology·2019

Wheat seedling cell walls show increasing arabinoxylan and decreasing beta-glucan during development. Ferulic acid dimers stabilize the wall during this transition, aiding structural changes.

Area of Science:

  • Plant Cell Wall Biology
  • Biochemistry
  • Developmental Biology

Background:

  • Arabinoxylan (AX) is a major non-cellulosic polysaccharide in cereal cell walls.
  • Understanding cell wall development is crucial for plant growth and biomass utilization.

Purpose of the Study:

  • To investigate changes in arabinoxylan content and composition during wheat seedling development.
  • To correlate these changes with enzyme activity and other cell wall components.

Main Methods:

  • Isolation and analysis of cell walls from wheat seedlings at different developmental stages.
  • Quantification of arabinoxylan, beta-glucan, and phenolic acids (ferulic and p-coumaric acid esters).
  • Analysis of ferulic acid dimers and their accumulation patterns.

Related Experiment Videos

Main Results:

  • Arabinoxylan content increased with seedling development, correlated with xylan synthase and arabinoxylan arabinosyltransferase activity.
  • beta-Glucan was degraded after day 4, preceding increased arabinoxylan deposition.
  • Ferulic acid esters and dimers increased significantly during lignification, with dimers peaking during the transition from beta-glucan to arabinoxylan dominance.
  • Spatial analysis within seedlings revealed similar developmental trends, with variations based on tissue type.

Conclusions:

  • Arabinoxylan accumulation and modification are key events in wheat seedling cell wall development.
  • beta-Glucan degradation precedes significant arabinoxylan deposition.
  • Ferulic acid dimers play a role in stabilizing the cell wall during the transition from a flexible to a rigid structure, particularly during lignification.