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Updated: Sep 30, 2026

Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
Published on: September 9, 2021
Activation of nucleolar DNA expression in hepatocytes by glucocorticoids and high density lipoproteins
Lev E Panin1, Valery F Maksimov, Ivan F Usynin
1Siberian Division of the Russian Academy of Medical Sciences, Institute of Biochemistry, Novosibirsk, Russia. ibch@cyber.ma.nsc.ru
Abstract:
A novel mechanism of protein biosynthesis regulation in liver under the action of reduced forms of steroid hormones (tetrahydrocortisol) and apolipoprotein A-I (apoA-I) is presented. Kupffer cells play an important role in uptake of the cortisol and high density lipoproteins (HDL) as well as in formation of the active complex, tetrahydrocortisol+apolipoprotein A-I (THC-apoA-I). If macrophages are stimulated by lipopolysaccharides (LPS), these processes enhance dramatically, thus causing parallel activation of nucleolar DNA expression and ribosome formation in hepatocytes. THC-apoA-I complex accelerates protein biosynthesis in primary cultures of hepatocytes, but not in macrophages and endotheliocytes.
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