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Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle

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Immunization of cattle with synthetic peptides derived from the Boophilus microplus gut protein (Bm86).

J H Patarroyo¹, R W Portela, R O De Castro

¹Laboratory of Biology and Control of Haematozoa, BIOAGRO/Veterinary Department, Federal University of Viçosa, 36571-000, Viçosa, MG Brazil. jpatarro@mail.ufv.br

Veterinary Immunology and Immunopathology

|July 20, 2002

Summary

Synthetic peptides targeting the Bm86 glycoprotein show high efficacy in controlling cattle ticks (Boophilus microplus). SBm7462 peptide demonstrated 81.05% efficacy, reducing tick numbers and reproductive capacity.

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Published on: January 31, 2018

Area of Science:

Veterinary parasitology
Immunology
Molecular biology

Background:

Cattle ticks, particularly Boophilus microplus, cause significant economic losses in livestock farming.
The Bm86 glycoprotein is a target for anti-tick vaccines.
Development of effective and sustainable tick control strategies is crucial for animal health and agricultural productivity.

Purpose of the Study:

To evaluate the efficacy of three synthetic peptides derived from the Bm86 glycoprotein in controlling Boophilus microplus infestations in cattle.
To assess the immune response elicited by these synthetic peptides.

Main Methods:

Three synthetic peptides (SBm4912, SBm7462, SBm19733) were synthesized based on the Bm86 glycoprotein.
Cattle were immunized subcutaneously with peptides and saponin adjuvant at 30-day intervals.
Immune response was measured by detecting anti-Bm86 specific antibodies (IgG) using in situ and Western blotting.
Immunized cattle were challenged with Boophilus microplus ticks, and tick parameters (number, weight, oviposition) were analyzed.

Main Results:

Immunization with synthetic peptides elicited an immune response characterized by anti-Bm86 specific antibodies.
Tick challenge revealed a reduction in the number, weight, and oviposition capacity of engorged females that fed on immunized cattle.
The SBm7462 synthetic peptide demonstrated significantly higher efficacy (81.05%) compared to other peptides (p<0.01).

Conclusions:

Synthetic peptides derived from Bm86 are effective in controlling cattle tick (Boophilus microplus) infestations.
The SBm7462 peptide shows high potential as a component of a vaccine for cattle tick control.
This approach offers a promising strategy for sustainable management of tick populations in cattle.