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Spatial distribution of mDLG6 mRNA in embryonic and adult mouse brain
Hidetoshi Inagaki1, Kimiko Tanaka, Mayuko Takada
1Molecular Neurophysiology Group, Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology, 1-1 Higashi, Tukuba, Ibaragi, 305-8566, Japan. hinagaki@aist.go.jp
Abstract:
We have isolated mouse DLG6 (mDLG6) cDNA clones by RT-PCR and then by using the RT-PCR products to screen a mouse brain cDNA library. The deduced amino acid sequence of mDLG6 shows 79.2% and 82.7% overall identity to human (hDLG6) and rat DLG6 (rDLG6), respectively. In situ hybridization revealed that mDLG6 mRNA is predominantly expressed in embryonic and adult brain.
Insights
Researchers isolated mouse DLG6 (mDLG6) cDNA, revealing high sequence identity to human and rat DLG6. Mouse DLG6 mRNA is primarily found in the brain during embryonic and adult stages.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- DLG6 is a member of the Discs large homolog family, known for its role in cell polarity and scaffolding.
- Understanding the expression patterns of DLG6 in different species is crucial for elucidating its function.
- Previous studies have characterized human and rat DLG6, but mouse DLG6 requires detailed investigation.
Purpose of the Study:
- To isolate and characterize mouse DLG6 (mDLG6) cDNA.
- To determine the sequence identity of mDLG6 compared to its human and rat orthologs.
- To investigate the expression pattern of mDLG6 mRNA in the mouse brain.
Main Methods:
- Complementary DNA (cDNA) isolation using Reverse Transcription Polymerase Chain Reaction (RT-PCR).
- Screening of a mouse brain cDNA library utilizing RT-PCR products.
- In situ hybridization to determine mRNA expression localization.
Main Results:
- Successfully isolated mouse DLG6 (mDLG6) cDNA clones.
- mDLG6 shares high overall amino acid identity with human DLG6 (79.2%) and rat DLG6 (82.7%).
- In situ hybridization confirmed predominant expression of mDLG6 mRNA in both embryonic and adult mouse brain tissues.
Conclusions:
- Mouse DLG6 is a conserved protein with high sequence similarity to human and rat orthologs.
- The expression of mDLG6 in the brain suggests a significant role in neural development and function.
- This study provides a foundation for further research into the specific functions of mDLG6 in the mammalian nervous system.