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Expression and evolution studies of ets genes in a primitive coelomate, the polychaete annelid, Hediste (Nereis)
Béatrice Bocquet-Muchembled1, Régine Leroux, Anne Chotteau-Lelièvre
1Laboratoire ELICO (Ecosystèmes Littoraux et Côtiers), groupe d'Ecologie Moléculaire, CNRS UPRES-A 8013, Bât. SN3, U.S.T.L., 59655 d'Ascq Cedex, Villeneuve, France.
Abstract:
The Ets family includes numerous proteins with a highly conserved DNA-binding domain of 85 amino acids named the ETS domain. Phylogenetic analyses from ETS domains revealed that this family could be divided into 13 groups, among them are ETS and ERG. The ets genes are present in the Metazoan kingdom and we have previously characterized the Nd ets and Nd erg genes in the polychaete annelid Hediste diversicolor. Here, we isolated a fragment encoding the ETS domain from Nd Ets, by genomic library screening. By Northern blot analysis, we showed that this gene was transcribed as one major mRNA of 2.6 kb and one minor mRNA of 3.2 kb. By in situ hybridization, we observed that Nd ets was expressed in the intestine and oocytes and that Nd erg was expressed in cellular clumps present in the coelomic cavity, in an area of proliferating cells situated between the last metamere and the pygidium. Finally, we showed that Nd erg shared the expression pattern of Nd ets in oocytes. Molecular modeling studies have revealed that the spatial structure of ETS domain of Nd Ets and Nd Erg was conserved, in comparison to the murine Ets-1 and human Fli-1 proteins, respectively.