Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Platelet aggregation by isolated and aggregated human IgG.

T Palosuo, J Leikola

    Clinical and Experimental Immunology
    |May 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The use of patient specific polyetheretherketone implants for reconstruction of maxillofacial deformities.

    Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery·2019
    Same author

    Implications of crop model ensemble size and composition for estimates of adaptation effects and agreement of recommendations.

    Agricultural and forest meteorology·2019
    Same author

    Mechanical analysis of cranial distractor attachment with three different resorbable fixation systems.

    Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery·2018
    Same author

    Force measurements during posterior calvarial vault osteodistraction: A novel measurement method.

    Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery·2017
    Same author

    Promoting ossification of calvarial defects in craniosynostosis surgery by demineralized bone plate and bone dust in different age groups.

    Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2016
    Same author

    Perinatal features and rate of cesarean section in newborns with non-syndromic sagittal synostosis.

    Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery·2016

    Bis-diazotized benzidine (BDB)-aggregated human IgG subclasses showed varying abilities to aggregate platelets. IgG1 and IgG3 were significantly more potent inducers of platelet aggregation (Pl.A.) than IgG2 and IgG4.

    Area of Science:

    • Immunology
    • Hematology
    • Protein Chemistry

    Background:

    • Human IgG antibodies exist in four subclasses (IgG1-IgG4).
    • Platelet aggregation is a critical process in hemostasis and thrombosis.
    • Myeloma proteins are monoclonal antibodies produced by plasma cells.

    Purpose of the Study:

    • To investigate the differential ability of human IgG subclasses, aggregated by bis-diazotized benzidine (BDB), to induce platelet aggregation (Pl.A.).
    • To compare the sensitivity of detection for BDB-aggregated IgG subclasses using the platelet aggregation test.

    Main Methods:

    • Isolation of human IgG subclass myeloma proteins.
    • Aggregation of IgG proteins using bis-diazotized benzidine (BDB).
    • Assessment of platelet aggregation (Pl.A.) using a sensitive sedimentation pattern test.

    Related Experiment Videos

    Main Results:

    • BDB-aggregated IgG1 and IgG3 induced platelet aggregation at concentrations as low as 0.1 mug/ml.
    • Significantly higher concentrations (200-1000 times) of BDB-aggregated IgG2 and IgG4 were required to induce Pl.A.
    • The observed reaction patterns shared similarities with complement fixation tests.

    Conclusions:

    • Human IgG1 and IgG3 subclasses are potent inducers of platelet aggregation when aggregated by BDB.
    • IgG2 and IgG4 subclasses exhibit markedly reduced capacity to induce platelet aggregation.
    • The platelet aggregation test is a sensitive method for detecting differences in the functional activity of aggregated IgG subclasses.