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Updated: Aug 14, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 18, 2010
Regulating cysteine protease activity: essential role of protease inhibitors as guardians and regulators
Boris Turk1, Dusan Turk, Guy S Salvesen
1Department of Biochemistry and Molecular Biology, J. Stefan Institute, Ljubljana, Slovenia. boris.turk@ijs.si
Abstract:
Cysteine proteases are widespread in nature. Their implication in numerous vital processes and pathologies make them highly attractive targets for drug design. The proper functioning and regulation of activity of cysteine proteases is a delicate balance of many factors, one of the most crucial being the protease inhibitors. In this review the basic principles of physiological protease inhibition by protein inhibitors are discussed with the focus on papain-like lysosomal cysteine proteases and the caspases, and their inhibitors.
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