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[Expression of insulin-like growth factor-I in bovine trabecular meshwork cells in vitro]
Yang Cao1, Jihua Dong, Houren Wei
1Department of Ophthalmology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ytsao@sohu.com
Objective:
To determine whether cultured bovine trabecular meshwork cells in vitro would express insulin-like growth factor-I (IGF-I).
Methods:
Newborn bovine trabecular meshwork cells of the third passage were cultured in vitro. The whole RNA from 10(6) cells was extracted with Trizol reagent, and specific oligonucleotide primer pair and reverse transcription polymerase chain reaction (RT-PCR) were used for detection of IGF-I messenger RNA. Immunohistochemical stain was used to detect IGF-I protein.
Results:
Cultured cells had the specific characteristics of bovine trabecular meshwork cells. A single RT-PCR amplified product whose sequence was homologous to the known sequence was obtained. IGF-I immunostain was positive.
Conclusions:
Trabecular meshwork cells may produce IGF-I. This finding strongly supports the possibility that IGF-I may affect trabecular meshwork cells through paracrine and autocrine mechanisms.