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Updated: Sep 30, 2026

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
10:53

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents

Published on: July 3, 2013

Changes of sarcoplamic reticular Ca(2+)-ATPase and IP(3)-I receptor mRNA expression in patients with atrial

Kejiang Cao1, Xiaojie Xia, Qijun Shan

  • 1Department of Cardiology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China.

Chinese Medical Journal
|July 23, 2002
PubMed
Abstract

Insights

Sarcoplasmic Reticular Ca(2+)-ATPase (SERCA) mRNA levels were lower and IP(3)-I receptors (IP(3)R(1)) mRNA levels were higher in patients with atrial fibrillation (AF). These expression changes may contribute to AF development and maintenance.

Area of Science:

  • Cardiology
  • Molecular Biology
  • Biochemistry

Background:

  • Atrial fibrillation (AF) is a common arrhythmia.
  • Alterations in calcium handling are implicated in AF pathophysiology.
  • Specific molecular targets like SERCA and IP3R1 warrant investigation.

Purpose of the Study:

  • To examine messenger RNA (mRNA) expression levels of SERCA and IP(3)R(1) in patients with AF.
  • To correlate these expression changes with the presence and duration of AF.

Main Methods:

  • Studied 38 patients with mitral stenosis undergoing cardiac surgery.
  • Quantified SERCA and IP(3)R(1) mRNA levels in atrial tissue using RT-PCR.
  • Normalized mRNA levels to GAPDH.

Main Results:

  • SERCA mRNA expression was significantly lower in AF patients compared to sinus rhythm.
  • IP(3)R(1) mRNA expression was significantly higher in AF patients.
  • Sustained AF duration correlated with increased IP(3)R(1) mRNA levels.

Conclusions:

  • Altered SERCA and IP(3)R(1) mRNA expression are associated with AF.
  • These molecular changes may play a role in the initiation or perpetuation of atrial fibrillation.

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