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Related Experiment Videos

Development of detection methods for ruminant interleukin (IL)-12.

J C Hope1, L S Kwong, G Entrican

  • 1Institute for Animal Health, Compton, Near Newbury, UK. Jayne.Hope@BBSRC.ac.uk

Journal of Immunological Methods
|July 23, 2002
PubMed
Summary

Researchers developed a sensitive ELISA to detect bovine interleukin-12 (IL-12), a key cytokine in animal immunology. This new method allows for better studies into the role of IL-12 in animal diseases.

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Area of Science:

  • Veterinary Immunology
  • Cytokine Research
  • Animal Disease Pathogenesis

Background:

  • Interleukin-12 (IL-12) is a critical cytokine in immune responses.
  • Detecting bovine IL-12 is essential for understanding ruminant immunology and disease.
  • Existing detection methods for bovine IL-12 are limited.

Purpose of the Study:

  • To develop and validate novel monoclonal antibodies (mAb) for detecting bovine IL-12.
  • To create a sensitive and specific assay for quantifying bovine IL-12.
  • To investigate the role of IL-12 in bovine immune responses and disease.

Main Methods:

  • Transient expression of recombinant bovine IL-12 (rbo IL-12) in COS-7 cells.
  • Generation and characterization of monoclonal antibodies against rbo IL-12.

Related Experiment Videos

  • Development of a sensitive luminometric ELISA for detecting bovine IL-12.
  • Detection of IL-12 in dendritic cell cultures and cytoplasmic staining.

    • Main Results:

    • Monoclonal antibodies were generated that specifically react with rbo IL-12.
    • A pair of mAb was identified that detects both recombinant and natural bovine IL-12 via ELISA.
    • A sensitive luminometric ELISA detected native bovine IL-12 in dendritic cell supernatants.
    • Bovine IL-12 was detected intracellularly in bovine dendritic cells.

    Conclusions:

    • A novel, sensitive ELISA for detecting bovine IL-12 was successfully developed.
    • The developed assay enables detection of IL-12 in various biological samples, including dendritic cells.
    • This advancement facilitates crucial studies on the role of IL-12 in animal immunology and disease pathogenesis.