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Identification and characterization of a baculovirus from Lonomia obliqua (Lepidoptera: Saturniidae)
José Luiz C Wolff1, Roberto H P Moraes, Elliot Kitajima
1Laboratório de Virologia Molecular, Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Av. Dr. Cândido, Xavier de Almeida, Souza, 200, 08780-911, Mogi das Cruzes, SP, Brazil. wolff@umc.br
Abstract:
A baculovirus has been isolated from larvae of Lonomia obliqua, a Saturniidae of medical importance due to a potent toxin found in their spines. Electron Microscopy analysis of the occlusion body obtained from diseased larvae showed polyhedra of approximately 1 microm in diameter containing multiple nucleocapsids per envelope. This baculovirus was thus named Lonomia obliqua multicapsid nucleopolyhedrovirus (LoobMNPV). Restriction endonuclease profiles of viral DNA digested with three restriction enzymes were obtained and the genome size was estimated to be 95.52 +/- 2.3 kbp. The polyhedrin gene of LoobMNPV was identified and its DNA sequence was determined. Phylogenetic analysis of the polyhedrin gene showed that the LoobMNPV polyhedrin belongs to group I NPV and that it is closely related to the polyhedrin of the NPV of Amsacta albistriga.