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Cloning and complete sequence of a novel HLA-A null allele, A*0253 N, with a termination codon generated by a C to G
1Shenzhen Institute of Transfusion Medicine, Shenzhen, China National Institutes of Health, Bethesda, USA.
Abstract:
A novel HLA-A null allele, A*0253 N, has been identified in two generations of a Chinese family using combined serological and molecular cloning approaches. Full-length genomic DNA sequencing indicated that this new allele differs from HLA-A*02011 by a single C to G substitution at nucleotide position 324 in exon 2. This mutation results in an amino acid change from a tyrosine codon to a stop codon at position 108. A PCR-SSP based method was developed to distinguish A*0253 N from A*02 alleles. No further individuals of A*0253 N were found in 718 Chinese blood donors who carry the HLA-A*02 allele1.
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